Mlodzianoski, Michael J.Cheng-Hathaway, Paul J.Bemiller, Shane M.McCray, Tyler J.Liu, ShengMiller, David A.Lamb, Bruce T.Landreth, Gary E.Huang, Fang2019-07-012019-07-012018-08Mlodzianoski, M. J., Cheng-Hathaway, P. J., Bemiller, S. M., McCray, T. J., Liu, S., Miller, D. A., … Huang, F. (2018). Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections. Nature methods, 15(8), 583–586. doi:10.1038/s41592-018-0053-8https://hdl.handle.net/1805/19790Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-μm-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer's disease.en-USPublisher PolicyAlzheimer DiseaseMembrane GlycoproteinsPlaque -- AmyloidReceptors -- ImmunologicArticle