Manicke, Nicholas E.Bills, Brandon J.Zhang, Chengsen2017-08-182017-08-182016-03Manicke, N. E., Bills, B. J., & Zhang, C. (2016). Analysis of biofluids by paper spray MS: advances and challenges. Bioanalysis, 8(6), 589–606. https://doi.org/10.4155/bio-2015-0018https://hdl.handle.net/1805/13872Abstract Paper spray MS is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma and urine spots at the low ng/ml to pg/ml levels without sample preparation. No front end separation is performed, so MS/MS or high-resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique and discuss some of its limitations.enPublisher Policyambient ionizationchromatographydried blood spotsArticle