Riley, Jonathan P.Kulkarni, AishwaryaMehrotra, PurviKoh, ByungheePerumal, Narayanan B.Kaplan, Mark H.Goenka, Shreevrat2025-04-242025-04-242013-12-20Riley JP, Kulkarni A, Mehrotra P, et al. PARP-14 binds specific DNA sequences to promote Th2 cell gene expression. PLoS One. 2013;8(12):e83127. Published 2013 Dec 20. doi:10.1371/journal.pone.0083127https://hdl.handle.net/1805/47412PARP-14, a member of the poly ADP-ribose polymerase super family, promotes T helper cell 2 (Th2) differentiation by regulating interleukin-4 (IL-4) and STAT6-dependent transcription. Yet, whether PARP-14 globally impacts gene regulation has not been determined. In this report, using an RNA pol II ChIP-seq approach, we identify genes in Th2 cells that are regulated by PARP-14, and either dependent or independent of ADP-ribosyltransferase catalytic activity. Our data demonstrate that PARP-14 enhances the expression of Th2 genes as it represses the expression of Th1-associated genes. Among the relevant targets are Signal Transducer and Activator of Transcription genes required for polarizing Th1 and Th2 cells. To define a mechanism for PARP-14 function, we use an informatics approach to identify putative PARP-14 DNA binding sites. Two putative PARP-14 binding motifs are identified in multiple Th2 cytokine genes, and we demonstrate that PARP-14 interacts with each motif using in vitro binding assays. Taken together our results indicate that PARP-14 is an important factor for T helper cell differentiation and it binds to specific DNA sequences to mediate its function.en-USAttribution 4.0 InternationalCytokinesNucleotide motifsGene expression profilingInterleukin-4DNAArticle