Lee, Young SooChang, Po-ChengHsueh, Chia-HsiangMaruyama, MitsunoriPark, Hyung WookRhee, Kyoung-SukHsieh, Yu-ChengShen, ChangyuWeiss, James N.Chen, ZhenhuiLin, Shien-FongChen, Peng-Sheng2015-11-022015-11-022013-10-24Lee, Y. S., Chang, P.-C., Hsueh, C.-H., Maruyama, M., Park, H. W., Rhee, K.-S., … Chen, P.-S. (2013). Apamin-Sensitive Calcium-Activated Potassium Currents in Rabbit Ventricles with Chronic Myocardial Infarction. Journal of Cardiovascular Electrophysiology, 24(10), 1144–1153. http://doi.org/10.1111/jce.12176https://hdl.handle.net/1805/7315Introduction Apamin-sensitive small-conductance calcium-activated potassium current (IKAS) is increased in heart failure. It is unknown if myocardial infarction (MI) is also associated with an increase of IKAS. Methods and Results We performed Langendorff perfusion and optical mapping in 6 normal hearts and 10 hearts with chronic (5 weeks) MI. An additional 6 normal and 10 MI hearts were used for patch clamp studies. The infarct size was 25% [95% confidence interval, 20 to 31] and the left ventricular ejection fraction was 0.5 [0.46 to 0.54]. The rabbits did not have symptoms of heart failure. The action potential duration measured to 80% repolarization (APD80) in the peri-infarct zone (PZ) was150 [142 to 159] ms, significantly (p=0.01) shorter than in the normal ventricles (158 to 177] ms). The intracellular Ca transient duration was also shorter in the PZ (148 [139 to 157] ms) than in normal ventricles (168 [157 to 180] ms; P=0.017). Apamin prolonged the APD80 in PZ by 9.8 [5.5 to 14.1] %, which is greater than in normal ventricles (2.8 [1.3 to 4.3] %, p=0.006). Significant shortening of APD80 was observed at the cessation of rapid pacing in MI but not in normal ventricles. Apamin prevented postpacing APD80 shortening. Patch clamp studies showed that IKAS was significantly higher in the PZ cells (2.51 [1.55 to 3.47] pA/pF, N=17) than in the normal cells (1.08 [0.36 to 1.80] pA/pF, N=15, p=0.019). Conclusion We conclude that IKAS is increased in MI ventricles and contributes significantly to ventricular repolarization especially during tachycardia.en-USPublisher Policyaction potentialsintracellular calciumion channelsrepolarization reservepotassium currentsmyocardial infarctionArticle