Cassidy, AnnelisePelletier, Stephane2023-10-242023-10-242023Cassidy A, Pelletier S. CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice. STAR Protoc. 2023;4(1):102116. doi:10.1016/j.xpro.2023.102116https://hdl.handle.net/1805/36597In this protocol, we describe the generation of conditional alleles in mice using the DECAI (DEgradation based on Cre-regulated Artificial Intron) approach. We detail steps for the CRISPR-mediated insertion of the short DECAI cassette within exon 3 of Scyl1 and the functional validation of alleles at genomic, transcriptomic, and protein levels. This strategy simplifies the process of generating mice with conditional alleles.en-USAttribution-NonCommercial-NoDerivatives 4.0 InternationalDevelopmental biologyGeneticsModel OrganismsMolecular BiologyCRISPRArticle