Moussa-Tooks, Alexandra B.Hetrick, William P.Green, John T.2022-04-212022-04-212020-12Moussa-Tooks AB, Hetrick WP, Green JT. Differential effects of two early life stress paradigms on cerebellar-dependent delay eyeblink conditioning. Neurobiol Stress. 2020;13:100242. Published 2020 Jul 17. doi:10.1016/j.ynstr.2020.100242https://hdl.handle.net/1805/28676Early life stress paradigms have become prominent in the animal literature to model atypical development. Currently, two models have prevailed within the literature: (1) limited bedding or nesting and (2) maternal separation or deprivation. Both models have produced aberrations spanning behavior and neural circuitry. Surprisingly, these two models have yet to be directly compared. The current study utilized delay eyeblink conditioning, an associative learning task with a well-defined cerebellar circuit, to compare the behavioral effects of standard limited bedding (postnatal day 2–9, n = 15) and maternal separation (60 min per day during postnatal day 2–14, n = 13) early life stress paradigms. Animals in all groups exhibited robust learning curves. Surprisingly, facilitated conditioning was observed in the maternal separation group. Rats that underwent limited bedding did not differ from the control or maternal separation groups on any conditioning measures. This study contributes to a clearer understanding of early life stress paradigms and the claims made about their mechanisms, which if better clarified can be properly leveraged to increase translational value.en-USAttribution 4.0 InternationalEarly life stressEyeblink conditioningLimited beddingMaternal separationExtinctionAssociative learningArticle