Liu, XiaowenYue, ZongliangYu, JeffreyDaguindau, EtienneKushekhar, KushiZhang, QingOgata, YukoGafken, Philip R.Inamoto, YoshihiroGracon, AdamWilkes, David S.Hansen, John A.Lee, Stephanie J.Chen, Jake YuePaczesny, Sophie2018-02-262018-02-262016-08Liu, X., Yue, Z., Yu, J., Daguindau, E., Kushekhar, K., Zhang, Q., … Paczesny, S. (2016). Proteomic characterization reveals that MMP-3 correlates with bronchiolitis obliterans syndrome following allogeneic hematopoietic cell and lung transplantation. American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(8), 2342–2351. https://doi.org/10.1111/ajt.137501600-6135https://hdl.handle.net/1805/15274Improved diagnostic methods are needed for bronchiolitis obliterans syndrome (BOS), a serious complication after allogeneic hematopoietic cell transplantation (HCT) and lung transplantation. For proteins candidate discovery, we compared plasma pools from HCT transplantation recipients with: BOS at onset (n=12), pulmonary infection (n=16), chronic graft-versus-host disease without pulmonary involvement (n=15), and no chronic complications post-HCT (n=15). Pools were labeled with different tags [isobaric Tags for Relative and Absolute Quantification (iTRAQ)], and two software tools identified differentially expressed proteins (≥1.5-fold change). Candidate proteins were further selected using a six-step computational biology approach. The diagnostic value of the lead candidate, matrix metalloproteinase-3 (MMP-3), was evaluated by ELISA in plasma of a verification cohort (n=112) with and without BOS following HCT (n=76) or lung transplantation (n=36). MMP-3 plasma concentrations differed significantly between patients with and without BOS (AUC=0.77). Thus, MMP-3 represents a potential non-invasive blood test for diagnosis of BOS.en-USPublisher Policybasic (laboratory) research/sciencebiomarkerbone marrow/hematopoietic stem cell transplantationbronchiolitis obliterans (BOS)lung transplantation/pulmonologytranslational research/scienceArticle