Seo, Seung-YongCorson, Timothy W.2022-04-062022-04-062019Seo, S. Y., & Corson, T. W. (2019). Small molecule target identification using photo-affinity chromatography. Methods in enzymology, 622, 347–374. https://doi.org/10.1016/bs.mie.2019.02.028https://hdl.handle.net/1805/28407Identification of the protein targets of bioactive small molecules is a routine challenge in chemical biology and phenotype-based drug discovery. Recent years have seen an explosion of approaches to meeting this challenge, but the traditional method of affinity pulldowns remains a practical choice in many contexts. This technique can be used as long as an affinity probe can be synthesized, usually with a crosslinking moiety to enable photo-affinity pulldowns. It can be applied to varied tissue types and can be performed with minimal specialized equipment. Here, we provide our protocol for photo-affinity pulldown experiments, with notes on making this method generally applicable to varied target identification challenges.en-USPublisher PolicyTarget identificationOff-targetMass spectrometrySynthetic chemistryPhotoreactive groupArticle