Felix Gomez, Grace GomezLippert, FrankAndo, MasatoshiZandona, Andrea FerreiraEckert, George J.Gregory, Richard L.2018-07-262018-07-262018-03-21Felix Gomez, G. G., Lippert, F., Ando, M., Zandona, A. F., Eckert, G. J., & Gregory, R. L. (2018). Effect of Violet-Blue Light on Streptococcus mutans-Induced Enamel Demineralization. Dentistry Journal, 6(2), 6. https://doi.org/10.3390/dj6020006https://hdl.handle.net/1805/16822Background: This in vitro study determined the effectiveness of violet-blue light (405 nm) on inhibiting Streptococcus mutans-induced enamel demineralization. Materials and Methods: S. mutans UA159 biofilm was grown on human enamel specimens for 13 h in 5% CO2 at 37 °C with/without 1% sucrose. Wet biofilm was treated twice daily with violet-blue light for five minutes over five days. A six-hour reincubation was included daily between treatments excluding the final day. Biofilms were harvested and colony forming units (CFU) were quantitated. Lesion depth (L) and mineral loss (∆Z) were quantified using transverse microradiography (TMR). Quantitative light-induced fluorescence Biluminator (QLF-D) was used to determine mean fluorescence loss. Data were analyzed using one-way analysis of variance (ANOVA) to compare differences in means. Results: The results demonstrated a significant reduction in CFUs between treated and non-treated groups grown with/without 1% sucrose. ∆Z was significantly reduced for specimens exposed to biofilms grown without sucrose with violet-blue light. There was only a trend on reduction of ∆Z with sucrose and with L on both groups. There were no differences in fluorescence-derived parameters between the groups. Conclusions: Within the limitations of the study, the results indicate that violet-blue light can serve as an adjunct prophylactic treatment for reducing S. mutans biofilm formation and enamel mineral loss.en-USAttribution 3.0 United StatesStreptococcus mutansdental cariesphototherapyviolet-blue lightArticle