Dantsu, YuliyaZhang, YingZhang, Wen2023-04-072023-04-072021-06Dantsu, Y., Zhang, Y., & Zhang, W. (2021). Synthesis and Structural Characterization of 2′-Deoxy-2′-fluoro-l-uridine Nucleic Acids. Organic Letters, 23(13), 5007–5011. https://doi.org/10.1021/acs.orglett.1c014981523-7060, 1523-7052https://hdl.handle.net/1805/32294Despite the development of artificial l-RNA/DNA as therapeutic molecules, the in-depth investigation on their chemical modifications is still limited. Here, we synthesize a chemically derivatized 2′-deoxy-2′-fluoro-l-uridine building block and incorporate it into oligonucleotides. Our thermo-denaturization and enzymatic digestion experiments reveal their superior stability. Furthermore, one crystal structure of l-type fluoro-DNA is determined to characterize its handedness. Our results reveal the increase of l-helix stability by fluoro-modification and provide the foundation for its future functional application.en-USPublisher PolicyNucleic acidsBiopolymersCrystal structureGeneticsArticle