Christodoulides, AlexeiHall, AbigailAlves, Nathan J.2024-02-132024-02-132023-07-13Christodoulides A, Hall A, Alves NJ. Exploring microplastic impact on whole blood clotting dynamics utilizing thromboelastography. Front Public Health. 2023;11:1215817. Published 2023 Jul 13. doi:10.3389/fpubh.2023.1215817https://hdl.handle.net/1805/38449This study investigates the influence of microplastics on blood clotting. It addresses the lack of comprehensive research on the effects of microplastic size and surface modification on clotting dynamics in human whole blood. Thromboelastography was used to examine aminated (aPS), carboxylated (cPS), and non-functionalized (nPS) polystyrene particles with sizes of 50, 100, and 500  nm. Results show that cPS consistently activated the clotting cascade, demonstrating increased fibrin polymerization rates, and enhanced clot strength in a size and concentration-dependent manner. nPS had minimal effects on clotting dynamics except for 50  nm particles at the lowest concentration. The clotting effects of aPS (100  nm particles) resembled those of cPS but were diminished in the 500  nm aPS group. These findings emphasize the importance of microplastic surface modification, size, concentration, and surface area on in-vitro whole blood clotting dynamics.en-USAttribution 4.0 InternationalThromboelastographyMicroplasticsCoagulationThrombosisTEGPolystyreneNanoplasticsArticle