Wang, XiaofangTu, XiaolinMa, YufeiChen, JieSong, YangLiu, Guangliang2024-04-292024-04-292022Wang X, Tu X, Ma Y, Chen J, Song Y, Liu G. Wnt3a-induced ST2 decellularized matrix ornamented PCL scaffold for bone tissue engineering. BIOCELL. 2022;46(9):2089-2099. doi:10.32604/biocell.2022.020069https://hdl.handle.net/1805/40327The limited bioactivity of scaffold materials is an important factor that restricts the development of bone tissue engineering. Wnt3a activates the classic Wnt/β-catenin signaling pathway which effects bone growth and development by the accumulation of β-catenin in the nucleus. In this study, we fabricated 3D printed PCL scaffold with Wnt3a-induced murine bone marrow-derived stromal cell line ST2 decellularized matrix (Wnt3a-ST2-dCM-PCL) and ST2 decellularized matrix (ST2-dCM-PCL) by freeze-thaw cycle and DNase decellularization treatment which efficiently decellularized >90% DNA while preserved most protein. Compared to ST2-dCM-PCL, Wnt3a-ST2-dCM-PCL significantly enhanced newly-seeded ST2 proliferation, osteogenic differentiation and upregulated osteogenic marker genes alkaline phosphatase (Alp), Runx2, type I collagen (Col 1) and osteocalcin (Ocn) mRNA expression. After 14 days of osteogenic induction, Wnt3a-ST2-dCM-PCL promoted ST2 mineralization. These results demonstrated that Wnt3a-induced ST2 decellularized matrix improve scaffold materials’ osteoinductivity and osteoconductivity.en-USAttribution 4.0 InternationalWnt3aDecellularized matrixBone marrow stromal cellsOsteogenic differentiationBone tissue engineeringArticle