Kole, AyeeshikCao, YingchunHui, JieBolad, Islam A.Alloosh, MouhamadCheng, Ji-XinSturek, Michael2019-06-192019-06-192018-11-28Kole, A., Cao, Y., Hui, J., Bolad, I. A., Alloosh, M., Cheng, J.-X., & Sturek, M. (2018). Comparative Quantification of Arterial Lipid by Intravascular Photoacoustic-Ultrasound Imaging and Near-Infrared Spectroscopy-Intravascular Ultrasound. Journal of Cardiovascular Translational Research. https://doi.org/10.1007/s12265-018-9849-21937-5395https://hdl.handle.net/1805/19634Intravascular photoacoustic-ultrasound (IVPA-US) imaging and near-infrared spectroscopy-intravascular ultrasound (NIRS-IVUS) are two hybrid modalities that detect arterial lipid, with comparison necessary to understand the relative advantages of each. We performed in vivo and ex vivo IVPA-US imaging of the iliac arteries of Ossabaw swine with metabolic syndrome (MetS) and lean swine to investigate sensitivity for early-stage atherosclerosis. We repeated imaging ex vivo with NIRS-IVUS for comparison to IVPA-US and histology. Both modalities showed significantly greater lipid in MetS vs. lean swine, but only IVPA-US localized the lipid as perivascular. To investigate late-stage atherosclerosis, we performed ex vivo IVPA-US imaging of a human coronary artery with comparison to NIRS-IVUS and histology. Two advanced fibroatheromas were identified, with agreement between IVPA-measured lipid area and NIRS-derived lipid content. As confirmed histologically, IVPA-US has sensitivity to detect lipid content similar to NIRS-IVUS and provides additional depth resolution, enabling quantification and localization of lipid cores within plaques.en-USAttribution 3.0 United StatesAtherosclerosisIntravascular imagingPhotoacoustic imagingNear-infrared spectroscopyLipid core plaquePerivascular adipose tissueSwineArticle