D’Souza, Cheryl A.Zhao, Fei LindaLi, XujianXu, YanDunn, Shannon E.Zhang, Li2016-03-312016-03-312016D’Souza, C. A., Zhao, F. L., Li, X., Xu, Y., Dunn, S. E., & Zhang, L. (2016). OGR1/GPR68 Modulates the Severity of Experimental Autoimmune Encephalomyelitis and Regulates Nitric Oxide Production by Macrophages. PLOS ONE, 11(2), e0148439. http://doi.org/10.1371/journal.pone.01484391932-6203https://hdl.handle.net/1805/9133Ovarian cancer G protein-coupled receptor 1 (OGR1) is a proton-sensing molecule that can detect decreases in extracellular pH that occur during inflammation. Although OGR1 has been shown to have pro-inflammatory functions in various diseases, its role in autoimmunity has not been examined. We therefore sought to determine whether OGR1 has a role in the development of T cell autoimmunity by contrasting the development of experimental autoimmune encephalomyelitis between wild type and OGR1-knockout mice. OGR1-knockout mice showed a drastically attenuated clinical course of disease that was associated with a profound reduction in the expansion of myelin oligodendrocyte glycoprotein 35-55-reactive T helper 1 (Th1) and Th17 cells in the periphery and a reduced accumulation of Th1 and Th17 effectors in the central nervous system. We determined that these impaired T cell responses in OGR1-knockout mice associated with a reduced frequency and number of dendritic cells in draining lymph nodes during EAE and a higher production of nitric oxide by macrophages. Our studies suggest that OGR1 plays a key role in regulating T cell responses during autoimmunity.en-USCC-BYT cellsMacrophagesAntigen-presenting cellsEnzyme-linked immunoassaysCentral nervous systemCytokinesNitric oxideLymph nodesArticle