Li, FangjiaChen, AndyReeser, AndrewWang, YueFan, YaoLiu, ShengzhiZhao, XinyuPrakash, RahulKota, DivyaLi, Bai-YanYokota, HirokiLiu, Jing2019-04-092019-04-092019-04-04Li, F., Chen, A., Reeser, A., Wang, Y., Fan, Y., Liu, S., … Liu, J. (2019). Vinculin Force Sensor Detects Tumor-Osteocyte Interactions. Scientific Reports, 9(1), 5615. https://doi.org/10.1038/s41598-019-42132-x2045-2322https://hdl.handle.net/1805/18805This study utilized a Förster resonance energy transfer (FRET)-based molecular tension sensor and live cell imaging to evaluate the effect of osteocytes, a mechanosensitive bone cell, on the migratory behavior of tumor cells. Two cell lines derived from MDA-MB-231 breast cancer cells were transfected with the vinculin tension sensor to quantitatively evaluate the force in focal adhesions of the tumor cell. Tumor cells treated with MLO-A5 osteocyte-conditioned media (CM) decreased the tensile forces in their focal adhesions and decreased their migratory potential. Tumor cells treated with media derived from MLO-A5 cells exposed to fluid flow-driven shear stress (FFCM) increased the tensile forces and increased migratory potential. Focal adhesion tension in tumor cells was also affected by distance from MLO-A5 cells when the two cells were co-cultured, where tumor cells close to MLO-A5 cells exhibited lower tension and decreased cell motility. Overall, this study demonstrates that focal adhesion tension is involved in altered migratory potential of tumor cells, and tumor-osteocyte interactions decrease the tension and motility of tumor cells.en-USAttribution 3.0 United StatesFörster resonance energy transfer (FRET)mechanosensitive bone cellstumor cellstumor-osteocyte interactionsArticle