Lin, Yu-HungMinner, Daniel E.Herring, Vincent L.Naumann, Christoph A.2018-05-152018-05-152012-11Lin, Y.-H., Minner, D. E., Herring, V. L., & Naumann, C. A. (2012). Physisorbed Polymer-Tethered Lipid Bilayer with Lipopolymer Gradient. Materials, 5(11), 2243–2257. http://doi.org/10.3390/ma5112243https://hdl.handle.net/1805/16190Physisorbed polymer-tethered lipid bilayers consisting of phospholipids and lipopolymers represent an attractive planar model membrane platform, in which bilayer fluidity and membrane elastic properties can be regulated through lipopolymer molar concentration. Herein we report a method for the fabrication of such a planar model membrane system with a lateral gradient of lipopolymer density. In addition, a procedure is described, which leads to a sharp boundary between regions of low and high lipopolymer molar concentrations. Resulting gradients and sharp boundaries are visualized on the basis of membrane buckling structures at elevated lipopolymer concentrations using epifluorescence microscopy and atomic force microscopy. Furthermore, results from spot photobleaching experiments are presented, which provide insight into the lipid lateral fluidity in these model membrane architectures. The presented experimental data highlight a planar, solid-supported membrane characterized by fascinating length scale-dependent dynamics and elastic properties with remarkable parallels to those observed in cellular membranes.en-USAttribution 3.0 United StatesGradient bilayerLangmuir-Blodgett transferLipopolymerPolymer-tethered lipid membraneEpifluorescenceAtomic force microscopyBuckling structureArticle