Broxmeyer, Hal E.Cooper, ScottCapitano, Maegan L.2022-06-212022-06-212020-10Broxmeyer HE, Cooper S, Capitano ML. Enhanced collection of phenotypic and engrafting human cord blood hematopoietic stem cells at 4°C. Stem Cells. 2020;38(10):1326-1331. doi:10.1002/stem.3243https://hdl.handle.net/1805/29381The number of hematopoietic stem cells (HSCs) collected in cord blood (CB) at the birth of a baby is a limiting factor for efficacious use of CB in hematopoietic cell transplantation (HCT). We now demonstrate that collecting and processing of human CB at 4°C within minutes of the baby's birth results in significantly enhanced numbers of rigorously defined phenotypic HSC and self-renewing NSG immune-deficient mouse engrafting and SCID-repopulating cells. This was associated with decreased numbers of hematopoietic progenitor cells (HPC), as noted previously for hypoxia collected/processed cells blocking ambient air induced differentiation of HSC to HPC. We have thus defined a simple, cost-effective, means to collect increased numbers of CB HSC, of potential use for clinical CB HCT.en-USPublisher PolicyCord bloodHematopoietic stem cellsHematopoietic progenitor cellsHematopoietic cell transplantationArticle