Du, JianguangWang, QunZiegler, Steven F.Zhou, Baohua2019-08-272019-08-272018-06-29Du, J., Wang, Q., Ziegler, S. F., & Zhou, B. (2018). FOXP3 interacts with hnRNPF to modulate pre-mRNA alternative splicing. The Journal of biological chemistry, 293(26), 10235–10244. doi:10.1074/jbc.RA117.001349https://hdl.handle.net/1805/20594FOXP3 promotes the development and function of regulatory T cells mainly through regulating the transcription of target genes. RNA alternative splicing has been implicated in a wide range of physiological and pathophysiological processes. We report here that FOXP3 associates with heterogeneous nuclear ribonucleoprotein (hnRNP) F through the exon 2-encoded region of FOXP3 and the second quasi-RNA recognition motif (qRRM) of hnRNPF. FOXP3 represses the ability of hnRNPF to bind to its target pre-mRNA and thus modulates RNA alternative splicing. Furthermore, overexpression of mouse hnRNPF in in vitro-differentiated regulatory T cells (Tregs) reduced their suppressive function. Thus, our studies identify a novel mechanism by which FOXP3 regulates mRNA alternative splicing to modulate the function of regulatory T cells.en-USPublisher PolicyForkhead box P3 (FOXP3)Heterogeneous nuclear ribonucleoprotein (hnRNP)RNA splicingRNA-protein interactionImmunologyTregImmunosuppressionAlternative splicingArticle