Mishra, VibhorSingh, JasleenWang, FengZhang, YixiangFukudome, AkihitoTrinidad, Jonathan C.Takagi, YuichiroPikaard, Craig S.2022-08-022022-08-022021-03-30Mishra V, Singh J, Wang F, et al. Assembly of a dsRNA synthesizing complex: RNA-DEPENDENT RNA POLYMERASE 2 contacts the largest subunit of NUCLEAR RNA POLYMERASE IV. Proc Natl Acad Sci U S A. 2021;118(13):e2019276118. doi:10.1073/pnas.2019276118https://hdl.handle.net/1805/29713In plants, transcription of selfish genetic elements such as transposons and DNA viruses is suppressed by RNA-directed DNA methylation. This process is guided by 24-nt short-interfering RNAs (siRNAs) whose double-stranded precursors are synthesized by DNA-dependent NUCLEAR RNA POLYMERASE IV (Pol IV) and RNA-DEPENDENT RNA POLYMERASE 2 (RDR2). Pol IV and RDR2 coimmunoprecipitate, and their activities are tightly coupled, yet the basis for their association is unknown. Here, we show that an interval near the RDR2 active site contacts the Pol IV catalytic subunit, NRPD1, the largest of Pol IV's 12 subunits. Contacts between the catalytic regions of the two enzymes suggests that RDR2 is positioned to rapidly engage the free 3' ends of Pol IV transcripts and convert these single-stranded transcripts into double-stranded RNAs (dsRNAs).en-USAttribution 4.0 InternationalRNA-directed DNA methylationGene silencingRNA interferenceArticle