Vale, A.H.F.Nascimento, D.C.Pineros, A.R.Ferreira, R.G.Santos, J.D.Aragon, D.C.Cunha, F.Q.Ramalho, F.S.Alves-Filho, J.C.Carlotti, A.P.C.P.2023-07-122023-07-122022-05-27Vale AHF, Nascimento DC, Pineros AR, et al. Riboflavin did not provide anti-inflammatory or antioxidant effects in an experimental model of sepsis. Braz J Med Biol Res. 2022;55:e12107. Published 2022 May 27. doi:10.1590/1414-431X2022e12107https://hdl.handle.net/1805/34300We aimed to evaluate whether the administration of riboflavin to septic animals reduces inflammation, oxidative stress, organ dysfunction, and mortality. C57BL/6 mice, 6-8 weeks old, were allocated to the study group (polymicrobial sepsis induced by cecal ligation and puncture (CLP) + antibiotic + iv riboflavin), control (CLP + antibiotic + iv saline), or naïve (non-operated controls). Serum concentrations of alanine aminotransferase (ALT), creatine kinase-MB (CK-MB), urea, and creatinine, and markers of inflammation [interleukin (IL)-6, tumor necrosis factor (TNF)-α, keratinocyte-derived chemokine (KC), and macrophage inflammatory protein (MIP)-2)], and oxidative stress (malondialdehyde (MDA) were measured 12 h after the experiment. Animal survival rates were calculated after 7 days. Means between groups were compared using linear regression models adjusted under the Bayesian approach. No significant difference was observed between control and study groups in serum concentrations of IL-6 (95% credible interval) (-0.35 to 0.44), TNF-α (-15.7 to 99.1), KC (-0.13 to 0.05), MIP-2 (-0.84 to 0.06), MDA (-1.25 to 2.53), or ALT (-6.6 to 11.5). Serum concentrations of CK-MB (-145.1 to -30.1), urea (-114.7 to -15.1), and creatinine (-1.14 to -0.01) were higher in the study group. Survival was similar in both groups (P=0.8). Therefore, the use of riboflavin in mice undergoing sepsis induced by CLP did not reduce inflammation, oxidative stress, organ dysfunction, or mortality compared with placebo.en-USAttribution 4.0 InternationalSepsisRiboflavinInflammatory responseOxidative stressOrgan dysfunctionArticle