Cadar, Adrian G.Feaster, Tromondae K.Durbin, Matthew D.Hong, Charles C.2019-05-062019-05-062017-08-14Cadar, A. G., Feaster, T. K., Durbin, M. D., & Hong, C. C. (2017). Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique. Current protocols in stem cell biology, 42, 4A.14.1–4A.14.7. doi:10.1002/cpsc.32https://hdl.handle.net/1805/19136This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications.en-USPublisher PolicyMatrigelCardiachiPSC-CMsMyocytesStem cellsArticle