Tudares, Mauro A.Eckert, George J.Lippert, Frank2024-05-092024-05-092023-11-03Tudares MA, Eckert GJ, Lippert F. Effects of silver diamine fluoride on demineralization protection after a secondary acid challenge. J Appl Oral Sci. 2023;31:e20230244. Published 2023 Nov 3. doi:10.1590/1678-7757-2023-0244https://hdl.handle.net/1805/40598Objective: This investigation describes the effects of 5% sodium fluoride varnish and 38% silver diamine fluoride on demineralization protection of human enamel lesions of three different severities after a secondary acid challenge. Study design: Specimens underwent color and enamel surface microhardness change measurements after demineralization and treatment events. Transverse microradiography was conducted following the secondary demineralization. Results: After treatments, enamel surface microhardness change showed that 24-hour lesions treated with fluoride varnish had less rehardening than 24-hour lesions treated with silver diamine fluoride (p<0.05), whereas 144-hour lesions from both treatment groups showed a beneficial decrease in surface microhardness change that was markedly better in samples treated with silver diamine fluoride (p<0.05). After the secondary demineralization, 24- and 144-hour lesions treated with silver diamine fluoride showed a sustained beneficial decrease in enamel surface microhardness change when compared to fluoride varnish-treated samples of the corresponding lesion severity (p<0.05). Transverse microradiography showed no difference between fluoride varnish- and silver diamine fluoride-treated samples of any corresponding lesion severity, indicating that remineralization in both fluoride varnish- and silver diamine fluoride-treated samples was proportional to each other after a secondary acid challenge. Conclusions: Using silver diamine fluoride may have comparable benefits to fluoride varnish in mineral loss prevention.en-USAttribution 4.0 InternationalSilver diamine fluorideRemineralizationEnamelCariesTransverse microradiographyArticle