Monaghan, TanyaMullish, Benjamin H.Patterson, JordanWong, Gane KSMarchesi, Julian R.Xu, HuipingJilani, TahseenKao, Dina2019-01-252019-01-252018-09-05Monaghan, T., Mullish, B. H., Patterson, J., Wong, G. K., Marchesi, J. R., Xu, H., … Kao, D. (2018). Effective fecal microbiota transplantation for recurrent Clostridioides difficile infection in humans is associated with increased signalling in the bile acid-farnesoid X receptor-fibroblast growth factor pathway. Gut Microbes, 0(0), 1–7. https://doi.org/10.1080/19490976.2018.15066671949-0976https://hdl.handle.net/1805/18256The mechanisms of efficacy for fecal microbiota transplantation (FMT) in treating recurrent Clostridioides difficile infection (rCDI) remain poorly defined, with restored gut microbiota-bile acid interactions representing one possible explanation. Furthermore, the potential implications for host physiology of these FMT-related changes in gut bile acid metabolism are also not well explored. In this study, we investigated the impact of FMT for rCDI upon signalling through the farnesoid X receptor (FXR)-fibroblast growth factor (FGF) pathway. Herein, we identify that in addition to restoration of gut microbiota and bile acid profiles, FMT for rCDI is accompanied by a significant, sustained increase in circulating levels of FGF19 and reduction in FGF21. These FGF changes were associated with weight gain post-FMT, to a level not exceeding the pre-rCDI baseline. Collectively, these data support the hypothesis that the restoration of gut microbial communities by FMT for rCDI is associated with an upregulated FXR-FGF pathway, and highlight the potential systemic effect of FMT.en-USAttribution-NonCommercial-NoDerivs 3.0 United StatesMicrobiotafecal microbiota transplantation (FMT)recurrent Clostridium difficile infection (rCDI)bile acid metabolismfibroblast growth factor (FGF)19Article