Dantsu, YuliyaZhang, YingZhang, Wen2023-04-172023-04-172021-10Dantsu, Y., Zhang, Y., & Zhang, W. (2021). Synthesis of 2′-Deoxy-2′-fluoro-L-cytidine and Fluorinated L-Nucleic Acids for Structural Studies. ChemistrySelect, 6(39), 10597–10600. https://doi.org/10.1002/slct.2021032022365-6549, 2365-6549https://hdl.handle.net/1805/32454The unique properties of fluorine atom on nucleic acid backbone can offer stricking functional and structural features. In order to extend the biological applications of L-type nucleic acid, we chemically incorporate the fluoro-modification into 2′-position of L-cytidine, and obtain a series of fluoro-modified L-DNAs/ L-RNAs. Our melting study indicates that the 2′-fluoro-modification does not disrupt the thermostabilities of wild-type L-nucleic acids. Consistently, our X-ray crystal structure reveals that fluoro-moiety cause no structural perturbation, and the fluoro-L-cytidine forms the Watson-Crick base pair with L-guanosine virtually identical to nonmodified L-type CG pair. This fluoro-modified cytidine provides a useful biochemical strategy to investigate L-nucleic acid as advanced molecular therapy.en-USPublisher Policycrystal structurefluoride modificationL-nucleic acidnucleosidesArticle