Sorkhdini, ParandGregory, Richard L.Crystal, Yasmi O.Tang, QingLippert, Frank2020-10-222020-10-222020-08Sorkhdini, P., Gregory, R. L., Crystal, Y. O., Tang, Q., & Lippert, F. (2020). Effectiveness of in vitro primary coronal caries prevention with silver diamine fluoride—Chemical vs biofilm models. Journal of Dentistry, 99, 103418. https://doi.org/10.1016/j.jdent.2020.10341https://hdl.handle.net/1805/24150Objectives The main goal of this study was to investigate the effectiveness of SDF and its individual components, silver (Ag+) and fluoride (F−) ions, in preventing enamel demineralization using biofilm and chemical models. Methodes Polished human enamel specimens were assigned to five treatment groups (n = 18 per group): SDF (38 %); SDF followed by application of a saturated solution of potassium iodide (SDF + KI); silver nitrate (AgNO3; silver control, 253,900 ppm Ag+); potassium fluoride (KF; fluoride control, 44,800 ppm F); deionized water (DIW). Treatments were applied once to sound enamel. In the biofilm model, specimens were demineralized by aerobic overnight incubation using cariogenic bacteria isolated from human saliva in brain heart infusion supplemented with 0.2 % sucrose for three days. In the chemical model, enamel specimens were immersed in a demineralizing solution containing 0.1 M lactic acid, 4.1 mM CaCl2, 8.0 mM KH2PO4, 0.2 % Carbopol 907, pH adjusted to 5.0 for five days. Vickers surface microhardness was used to determine the extent of enamel demineralization. Data were analyzed using one-way ANOVA. Results In the chemical model, there was no statistically significant difference between SDF and SDF + KI in preventing coronal caries (p < 0.0001). In the biofilm model, SDF + KI was significantly less effective in preventing demineralization than SDF (p < 0.0001). In both models, SDF and SDF + KI were superior in their ability to prevent caries lesion formation than AgNO3 and DIW. Conclusion KI application after SDF treatment appears to impair SDF’s ability to prevent biofilm-mediated but not chemically induced demineralization.enPublisher Policysilver diamine fluoridedark stainingdemineralizationArticle