Pourafshar, ShirinNicchitta, MiraTyson, Crystal C.Svetkey, Laura P.Corcoran, David L.Bain, James R.Muehlbauer, Michael J.Ilkayeva, OlgaO’Connell, Thomas MLin, Pao-HwaScialla, Julia J.2022-12-092022-12-092021-05-22Pourafshar S, Nicchitta M, Tyson CC, et al. Urine and Plasma Metabolome of Healthy Adults Consuming the DASH (Dietary Approaches to Stop Hypertension) Diet: A Randomized Pilot Feeding Study. Nutrients. 2021;13(6):1768. Published 2021 May 22. doi:10.3390/nu13061768https://hdl.handle.net/1805/30712We aimed to identify plasma and urine metabolites altered by the Dietary Approaches to Stop Hypertension (DASH) diet in a post-hoc analysis of a pilot feeding trial. Twenty adult participants with un-medicated hypertension consumed a Control diet for one week followed by 2 weeks of random assignment to either Control or DASH diet. Non-missing fasting plasma (n = 56) and 24-h urine (n = 40) were used to profile metabolites using untargeted gas chromatography/mass spectrometry. Linear models were used to compare metabolite levels between the groups. In urine, 19 identifiable untargeted metabolites differed between groups at p < 0.05. These included a variety of phenolic acids and their microbial metabolites that were higher during the DASH diet, with many at false discovery rate (FDR) adjusted p < 0.2. In plasma, eight identifiable untargeted metabolites were different at p < 0.05, but only gamma-tocopherol was significantly lower on DASH at FDR adjusted p < 0.2. The results provide insights into the mechanisms of benefit of the DASH diet.en-USAttribution 4.0 InternationalNutritionPolyphenolsBlood pressureDASHMetabolomicsArticle