Andere, Anne A.Pimsler, Meaghan L.Tarone, Aaron M.Picard, Christine J.2023-08-082023-08-082020Andere, A. A., Pimsler, M. L., Tarone, A. M., & Picard, C. J. (2020). The genomes of a monogenic fly: Views of primitive sex chromosomes. Scientific Reports, 10(1), 15728. https://doi.org/10.1038/s41598-020-72880-0https://hdl.handle.net/1805/34797The production of male and female offspring is often determined by the presence of specific sex chromosomes which control sex-specific expression, and sex chromosomes evolve through reduced recombination and specialized gene content. Here we present the genomes of Chrysomya rufifacies, a monogenic blow fly (females produce female or male offspring, exclusively) by separately sequencing and assembling each type of female and the male. The genomes (> 25X coverage) do not appear to have any sex-linked Muller F elements (typical for many Diptera) and exhibit little differentiation between groups supporting the morphological assessments of C. rufifacies homomorphic chromosomes. Males in this species are associated with a unimodal coverage distribution while females exhibit bimodal coverage distributions, suggesting a potential difference in genomic architecture. The presence of the individual-sex draft genomes herein provides new clues regarding the origination and evolution of the diverse sex-determining mechanisms observed within Diptera. Additional genomic analysis of sex chromosomes and sex-determining genes of other blow flies will allow a refined evolutionary understanding of how flies with a typical X/Y heterogametic amphogeny (male and female offspring in similar ratios) sex determination systems evolved into one with a dominant factor that results in single sex progeny in a chromosomally monomorphic system.en-USAttribution 4.0 InternationalEntomologySexual selectionDipteraSex chromosomesArticle