Su, JingHuang, Lynn S.Barnard, RyanParks, GrahamCappellari, JamesBellinger, ChristinaDotson, TravisCraddock, LouPrakash, BharatHovda, JonathanClark, HollinsPetty, William JeffreyPasche, BorisChan, Michael D.Miller, Lance D.Ruiz, Jimmy2022-10-272022-10-272021-04-16Su J, Huang LS, Barnard R, et al. Comprehensive and Computable Molecular Diagnostic Panel (C2Dx) From Small Volume Specimens for Precision Oncology: Molecular Subtyping of Non-Small Cell Lung Cancer From Fine Needle Aspirates. Front Oncol. 2021;11:584896. Published 2021 Apr 16. doi:10.3389/fonc.2021.584896https://hdl.handle.net/1805/30421The Comprehensive, Computable NanoString Diagnostic gene panel (C2Dx) is a promising solution to address the need for a molecular pathological research and diagnostic tool for precision oncology utilizing small volume tumor specimens. We translate subtyping-related gene expression patterns of Non-Small Cell Lung Cancer (NSCLC) derived from public transcriptomic data which establish a highly robust and accurate subtyping system. The C2Dx demonstrates supreme performance on the NanoString platform using microgram-level FNA samples and has excellent portability to frozen tissues and RNA-Seq transcriptomic data. This workflow shows great potential for research and the clinical practice of cancer molecular diagnosis.en-USAttribution 4.0 InternationalNon-small cell lung cancerMolecular signatureFine needle aspirationLogistic regressionElastic net regularizationCancer subtypingArticle