Awe, OlufolakemiHufford, Matthew M.Wu, HaoPham, DuyChang, Hua-ChenJabeen, RukhsanaDent, Alexander L.Kaplan, Mark H.2016-12-212016-12-212015-10-15Awe, O., Hufford, M. M., Wu, H., Pham, D., Chang, H.-C., Jabeen, R., … Kaplan, M. H. (2015). PU.1 Expression in T Follicular Helper Cells Limits CD40L-Dependent Germinal Center B Cell Development. Journal of Immunology (Baltimore, Md.: 1950), 195(8), 3705–3715. https://doi.org/10.4049/jimmunol.15007800022-1767 1550-6606https://hdl.handle.net/1805/11690PU.1 is an ETS family transcription factor important for the development of multiple hematopoietic cell lineages. Previous work demonstrated a critical role for PU.1 in promoting Th9 development, and in limiting Th2 cytokine production. Whether PU.1 has functions in other T helper lineages is not clear. In this report we examined the effects of ectopic expression of PU.1 in CD4+T cells and observed decreased expression of genes involved with the function of T follicular helper (Tfh) cells, including Il21 and Tnfsf5 (encoding CD40L). T cells from conditional mutant mice that lack expression of PU.1 in T cells (Sfpi1lck−/−) demonstrated increased production of CD40L and IL-21 in vitro. Following adjuvant-dependent or adjuvant-independent immunization, we observed that Sfpi1lck−/− mice had increased numbers of Tfh cells, increased germinal center B cells, and increased antibody production in vivo. This correlated with increased expression of IL-21 and CD40L in Tfh cells from Sfpi1lck−/− mice, compared to control mice. Finally, although blockade of IL-21 did not affect germinal center B cells in Sfpi1lck−/− mice, anti-CD40L treatment of immunized Sfpi1lck−/− mice decreased germinal center B cell numbers and antigen-specific immunoglobulin concentrations. Together, these data indicate an inhibitory role of PU.1 in the function of T follicular helper cells, germinal centers, and Tfh-dependent humoral immunity.en-USPublisher PolicyB-LymphocytesCD40 LigandGerminal CenterProto-Oncogene ProteinsT-LymphocytesArticle