Scott, Jonathan M.Swallow, Elizabeth A.Metzger, Corinne E.Kohler, RachelWallace, Joseph M.Stacy, Alexander J.Allen, Matthew R.Gasier, Heath G.2024-06-052024-06-052022-10-28Scott, J. M., Swallow, E. A., Metzger, C. E., Kohler, R., Wallace, J. M., Stacy, A. J., Allen, M. R., & Gasier, H. G. (2022). Iron deficiency and high-intensity running interval training do not impact femoral or tibial bone in young female rats. British Journal of Nutrition, 128(8), 1518–1525. https://doi.org/10.1017/S0007114521004426https://hdl.handle.net/1805/41234In the US, as many as 20% of recruits sustain stress fractures during basic training. In addition, approximately one-third of female recruits develop iron deficiency upon completion of training. Iron is a cofactor in bone collagen formation and vitamin D activation, thus we hypothesized iron deficiency may be contributing to altered bone microarchitecture and mechanics during 12-weeks of increased mechanical loading. Three-week old female Sprague Dawley rats were assigned to one of four groups: iron adequate sedentary, iron deficient sedentary, iron adequate exercise, and iron deficient exercise. Exercise consisted of high-intensity treadmill running (54 min 3×/week). After 12-weeks, serum bone turnover markers, femoral geometry and microarchitecture, mechanical properties and fracture toughness, and tibiae mineral composition and morphometry were measured. Iron deficiency increased the bone resorption markers C-terminal telopeptide type I collagen and tartate-resistant acid phosphatase 5b (TRAcP 5b). In exercised rats, iron deficiency further increased bone TRAcP 5b, while in iron adequate rats, exercise increased the bone formation marker procollagen type I N-terminal propeptide. In the femur, exercise increased cortical thickness and maximum load. In the tibia, iron deficiency increased the rate of bone formation, mineral apposition, and zinc content. These data show that the femur and tibia structure and mechanical properties are not negatively impacted by iron deficiency despite a decrease in tibiae iron content and increase in serum bone resorption markers during 12-weeks of high-intensity running in young growing female rats.en-USPublisher Policynutritionironexerciserodentmicroarchitecturemechanical testingdynamic histomorphometryArticle