Wang, ExingMeier, Daniel J.Sandoval, Ruben M.Von Hendy-Willson, Vanessa E.Pressler, Barrak M.Bunch, Robert M.Alloosh, MouhamadSturek, Michael S.Schwartz, George J.Molitoris, Bruce A.2018-06-122018-06-122012-01Wang, E., Meier, D. J., Sandoval, R. M., Von Hendy-Willson, V. E., Pressler, B. M., Bunch, R. M., … Molitoris, B. A. (2012). A portable fiberoptic ratiometric fluorescence analyzer provides rapid point-of-care determination of glomerular filtration rate in large animals. Kidney International, 81(1), 112–117. https://doi.org/10.1038/ki.2011.2940085-2538https://hdl.handle.net/1805/16474Measurement of the glomerular filtration rate (GFR) is the gold standard for precise assessment of kidney function. A rapid, point-of-care determination of the GFR may provide advantages in the clinical setting over currently available assays. Here we demonstrate a proof of principle for such an approach in a pig and dogs, two species that approximate the vascular access and GFR results expected in humans. In both animal models, a sub-millimeter optical fiber that delivered excitation light and collected fluorescent emissions was inserted into a peripheral vein (dog) or central venous access (pig) by means of commercial intravenous catheters. A mixture of fluorescent chimeras of a small freely filterable reporter and large non-filterable plasma volume marker were infused as a bolus, excited by light-emitting diodes, and the in vivo signals detected and quantified by photomultiplier tubes in both species in less than 60 min. Concurrent standardized 6-h iohexol plasma kidney clearances validated the accuracy of our results for both physiologic and a chronic kidney disease setting. Thus, our ratiometric technique allows for both measurement of plasma vascular volume and highly accurate real-time GFR determinations, enabling clinical decision making in real time.en-USPublisher Policyacute kidney injurychronic kidney diseaseglomerular filtration rateArticle