Shaner, Nathan C.Lambert, Gerard G.Chammas, AndrewNi, YuhuiCranfill, Paula J.Baird, Michelle A.Sell, Brittney R.Allen, John R.Day, Richard N.Israelsson, MariaDavidson, Michael W.Wang, Jiwu2025-05-122025-05-122013Shaner NC, Lambert GG, Chammas A, et al. A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods. 2013;10(5):407-409. doi:10.1038/nmeth.2413https://hdl.handle.net/1805/47989We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.en-USPublisher PolicyAnimalsChordataGreen fluorescent proteinsMolecular sequence dataStochastic processesArticle