Wu, HaoXu, Lin-linTeuscher, PaullaLiu, HongKaplan, Mark H.Dent, Alexander L.2017-05-232017-05-232015-09Wu, H., Xu, L.-L., Teuscher, P., Liu, H., Kaplan, M. H., & Dent, A. L. (2015). An Inhibitory Role for the Transcription Factor Stat3 in Controlling IL-4 and Bcl6 Expression in Follicular Helper T cells. Journal of Immunology (Baltimore, Md. : 1950), 195(5), 2080–2089. http://doi.org/10.4049/jimmunol.1500335https://hdl.handle.net/1805/12695The transcription factor Bcl6 is required for the development of the follicular helper T (TFH) cells. Cytokines that activate Stat3 promote Bcl6 expression and TFH cell differentiation. Previous studies with an acute virus infection model showed that TFH cell differentiation was decreased but not blocked in the absence of Stat3. In this study, we further analyzed the role of Stat3 in TFH cells. In Peyer’s patches (PPs), we found that compared to wild-type, Stat3-deficient TFH cells developed at a 25% lower rate, and expressed increased IFNγ and IL-4. While PP germinal center B (GCB) cells developed at normal numbers with Stat3-deficient TFH cells, IgG1 class switching was greatly increased. Following immunization with Sheep Red Blood Cells (SRBC), splenic Stat3-deficient TFH cells developed at a slower rate than in control mice and splenic GCB cells were markedly decreased. Stat3-deficient TFH cells developed poorly in a competitive bone marrow chimera environment. Under all conditions tested, Stat3-deficient TFH cells over-expressed both IL-4 and Bcl6, a pattern specific for the TFH cell population. Finally, we found in vitro that repression of IL-4 expression in CD4 T cells by Bcl6 required Stat3 function. Our data indicate that Stat3 can repress the expression of Bcl6 and IL-4 in TFH cells, and that Stat3 regulates the ability of Bcl6 to repress target genes. Overall, we conclude that Stat3 is required to fine-tune the expression of multiple key genes in TFH cells, and that the specific immune environment determines the function of Stat3 in TFH cells.en-USPublisher PolicyCD4-Positive T-LymphocytesCell DifferentiationDNA-Binding ProteinsInterleukin-4Peyer's PatchesSTAT3 Transcription FactorSpleenT-Lymphocytes, Helper-InducerArticle