Amarsaikhan, NansalmaaStolz, Dylan J.Wilcox, AmberSands, Ethan M.Tsoggerel, AngarGravely, HaleyTempleton, Steven P.2019-08-202019-08-202019-05-10Amarsaikhan, N., Stolz, D. J., Wilcox, A., Sands, E. M., Tsoggerel, A., Gravely, H., & Templeton, S. P. (2019). Reciprocal Inhibition of Adiponectin and Innate Lung Immune Responses to Chitin and Aspergillus fumigatus. Frontiers in immunology, 10, 1057. doi:10.3389/fimmu.2019.01057https://hdl.handle.net/1805/20452Chitin is a structural biopolymer found in numerous organisms, including pathogenic fungi, and recognized as an immune-stimulating pathogen associated molecular pattern by pattern recognition molecules of the host immune system. However, programming and regulation of lung innate immunity to chitin inhalation in the context of inhalation of fungal pathogens such as Aspergillus fumigatus is complex and our understanding incomplete. Here we report that the systemic metabolism-regulating cytokine adiponectin is decreased in the lungs and serum of mice after chitin inhalation, with a concomitant decrease in surface expression of the adiponectin receptor AdipoR1 on lung leukocytes. Constitutive lung expression of acidic mammalian chitinase resulted in decreased inflammatory cytokine gene expression and neutrophil recruitment, but did not significantly affect lung adiponectin transcription. Exogenous recombinant adiponectin specifically dampened airway chitin-mediated eosinophil recruitment, while adiponectin deficiency resulted in increased airway eosinophils. The presence of adiponectin also resulted in decreased CCL11-mediated migration of bone marrow-derived eosinophils. In contrast to purified chitin, aspiration of viable conidia from the high chitin-expressing A. fumigatus isolate Af5517 resulted in increased neutrophil recruitment and inflammatory cytokine gene expression in adiponectin-deficient mice, while no significant changes were observed in response to the isolate Af293. Our results identify a novel role for the adiponectin pathway in inhibition of lung inflammatory responses to chitin and A. fumigatus inhalation.en-USAttribution-NonCommercial-NoDerivs 3.0 United StatesAspergillus fumigatusAdiponectinChitinEosinophilsInflammatory cytokinesLung immune responsesNeutrophilsArticle