Humphrey, JohnTriedman, MirandaNyandiko, WinstoneSang, EdwinKemboi, EmmanuelAlera, MarshaNovitsky, VladManne, AkarshJepkemboi, EslyneOrido, MillicentApondi, EdithVreeman, RachelWools-Kaloustian, KaraKantor, Rami2023-07-052023-07-052022-05-31Humphrey J, Triedman M, Nyandiko W, et al. A Challenging Knowledge Gap: Estimating Modes of HIV Acquisition Among Adolescents Entering HIV Care During Adolescence. Glob Pediatr Health. 2022;9:2333794X221101768. Published 2022 May 31. doi:10.1177/2333794X221101768https://hdl.handle.net/1805/34103Characterizing HIV acquisition modes among adolescents with HIV (AHIV) enrolling in care during adolescence is a challenging gap that impacts differential interventions. We explored whether primary data collection with targeted questionnaires may address this gap and improve understanding of risk factors and perceptions about adolescents' HIV acquisition, in Kenyan AHIV entering care at ≥10 years, and their mothers with HIV (MHIV). Clinical data were derived through chart review. Among 1073 AHIV in care, only 26 (2%) met eligibility criteria of being ≥10 years at care enrollment, disclosed to, and with living MHIV. Among 18/26 AHIV-MHIV dyads enrolled (median age of AHIV 14 years), none had documented HIV acquisition modes. Data suggested perinatal infection in 17/18 AHIV, with 1 reported non-perinatal acquisition risk factor, and some discordance between adolescent-mother perceptions of HIV acquisition. In this difficult-to-enroll, vulnerable population of AHIV-MHIV dyads, primary data collection can enhance understanding of AHIV acquisition modes.en-USAttribution-NonCommercial 4.0 InternationalHIVHIV transmission modeAdolescentLate care entryMother-to-child transmissionArticle