Kaplan, Mark H.Glosson, Nicole L.Blum, Janice Sherry, 1957-Yu, AndyHarrington, Maureen A.2015-02-112015-06-022014https://hdl.handle.net/1805/5902http://dx.doi.org/10.7912/C2/1736Indiana University-Purdue University Indianapolis (IUPUI)IL-17-producing T cells are critical to the development of pathogen and tumor immunity, but also contribute to the pathology of autoimmune diseases and allergic inflammation. CD8+ (Tc17) and CD4+ (Th17) IL-17-secreting T cells develop in response to a cytokine environment that activates Signal Transducer and Activator of Transcription (STAT) proteins, though the mechanisms underlying Tc17/Th17 development and stability are still unclear. In vivo, Tc17 cells clear vaccinia virus infection and acquire cytotoxic potential, that is independent of IL-17 production and the acquisition of IFN-γ-secreting potential, but partially dependent on Fas ligand, suggesting that Tc17-mediated vaccinia virus clearance is through cell killing independent of an acquired Tc1 phenotype. In contrast, memory Th cells and NKT cells display STAT4-dependent IL-23-induced IL-17 production that correlates with Il23r expression. IL-23 does not activate STAT4 nor do other STAT4-activating cytokines induce Il23r expression in these populations, suggesting a T cell-extrinsic role for STAT4 in mediating IL-23 responsiveness. Although IL-23 is important for the maintenance of IL-17-secreting T cells, it also promotes their instability, often resulting in a pathogenic Th1-like phenotype in vitro and in vivo. In vitro-derived Th17 cells are also flexible when cultured under polarizing conditions that promote Th2 or Th9 differentiation, adopting the respective effector programs, and decreasing IL-17 production. However, in models of allergic airway disease, Th17 cells do not secrete alternative cytokines nor adopt other effector programs, and remain stable IL-17-secretors. In contrast to Th1-biased pro-inflammatory environments that induce Th17 instability in vivo, during allergic inflammatory disease, Th17 cells are comparatively stable, and retain the potential to produce IL-17. Together these data document that the inflammatory environment has distinct effects on the stability of IL-17-secreting T cells in vivo.en-UST cell, IL-17, Th17, Tc17, STAT4, Allergic Airway DiseaseAutoimmune diseases -- Research -- Evaluation -- AnalysisAnti-inflammatory agentsInflammation -- Immunological aspectsRespiratory allergyT cells -- ImmunologyInterleukins -- ImmunologyAsthma -- PathophysiologyTh1 cells -- ResearchTh2 cells -- ResearchCellular immunityImmune response -- RegulationCellular control mechanismsThesis