Ren, HaiyanXu, ZhuwenZhang, WenhaoHuang, JianhuiChen, ShipingWang, LixinHan, Xingguo2015-02-242015-02-242013-10Ren, H., Xu, Z., Zhang, W., Jiang, L., Huang, J., Chen, S., ... & Han, X. (2013). Linking ethylene to nitrogen-dependent leaf longevity of grass species in a temperate steppe. Annals of botany, 112(9), 1879-1885. http://dx.doi.org/ 10.1093/aob/mct223https://hdl.handle.net/1805/5944Author's manuscript made available in accordance with the publisher's policy.Background and Aims Leaf longevity is an important plant functional trait that often varies with soil nitrogen supply. Ethylene is a classical plant hormone involved in the control of senescence and abscission, but its role in nitrogen-dependent leaf longevity is largely unknown. Methods Pot and field experiments were performed to examine the effects of nitrogen addition on leaf longevity and ethylene production in two dominant plant species, Agropyron cristatum and Stipa krylovii, in a temperate steppe in northern China. Key Results Nitrogen addition increased leaf ethylene production and nitrogen concentration but shortened leaf longevity; the addition of cobalt chloride, an ethylene biosynthesis inhibitor, reduced leaf nitrogen concentration and increased leaf longevity. Path analysis indicated that nitrogen addition reduced leaf longevity mainly through altering leaf ethylene production. Conclusions These findings provide the first experimental evidence in support of the involvement of ethylene in nitrogen-induced decrease in leaf longevity.en-USethylene evolutionInner Mongolialeaf longevityArticle