Ha, Tae WonKang, Hyun SooKim, Tae-HeeKwon, Ji HyunKim, Hyun KyuRyu, AeliJeon, HyejiHan, JaeseokBroxmeyer, Hal E.Hwang, YongsungLee, Yun KyungLee, Man Ryul2019-06-052019-06-052018-11-30Ha, T. W., Kang, H. S., Kim, T. H., Kwon, J. H., Kim, H. K., Ryu, A., … Lee, M. R. (). MiR-9 Controls Chemotactic Activity of Cord Blood CD34⁺ Cells by Repressing CXCR4 Expression. International journal of stem cells, 11(2), 187–195. doi:10.15283/ijsc18057https://hdl.handle.net/1805/19535Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. However, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34＋ cells as well as in 293T and TF-1 cell lines. Since CXCR4 is a specific receptor for the stromal cell derived factor-1 (SDF-1) chemotactic factor, we investigated whether sense miR-9 and antisense miR-9 influenced CXCR4-mediated chemotactic mobility of primary CB CD34＋ cells and TF-1 cells. Ectopic overexpression of sense miR-9 and antisense miR-9 respectively down- and up-regulated SDF-1-mediated chemotactic cell mobility. To our knowledge, this study is the first to report that miR-9 may play a role in regulating CXCR4 expression and SDF-1-mediated chemotactic activity of CB CD34＋ cells.en-USAttribution-NonCommercial-NoDerivs 3.0 United StatesCXCR4Cell migrationCord bloodmicroRNAArticle