Schaffer, Leah V.Millikin, Robert J.Miller, Rachel M.Anderson, Lissa C.Fellers, Ryan T.Ge, YingKelleher, Neil L.LeDuc, Richard D.Liu, XiaowenPayne, Samuel H.Sun, LiangliangThomas, Paul M.Tucholski, TrishaWang, ZheWu, SiWu, ZhijieYu, DahangShortreed, Michael R.Smith, Lloyd M.2020-07-202020-07-202019-05Schaffer, L. V., Millikin, R. J., Miller, R. M., Anderson, L. C., Fellers, R. T., Ge, Y., Kelleher, N. L., LeDuc, R. D., Liu, X., Payne, S. H., Sun, L., Thomas, P. M., Tucholski, T., Wang, Z., Wu, S., Wu, Z., Yu, D., Shortreed, M. R., & Smith, L. M. (2019). Identification and Quantification of Proteoforms by Mass Spectrometry. Proteomics, 19(10), e1800361. https://doi.org/10.1002/pmic.201800361https://hdl.handle.net/1805/23284A proteoform is a defined form of a protein derived from a given gene with a specific amino acid sequence and localized post-translational modifications. In top-down proteomic analyses, proteoforms are identified and quantified through mass spectrometric analysis of intact proteins. Recent technological developments have enabled comprehensive proteoform analyses in complex samples, and an increasing number of laboratories are adopting top-down proteomic workflows. In this review, we outline some recent advances and discuss current challenges and future directions for the field.en-USPublisher PolicyBioinformaticsMass spectrometryProteoformProteoform familyTop-down proteomicsArticle