Zimmerer, J. M.Swamy, P.Sanghavi, P. B.Wright, C. L.Abdel-Rasoul, M.Elzein, S. M.Brutkiewicz, R. R.Bumgardner, G. L.2016-10-252016-10-252014-11Zimmerer, J. M., Swamy, P., Sanghavi, P. B., Wright, C. L., Abdel-Rasoul, M., Elzein, S. M., … Bumgardner, G. L. (2014). Critical role of NKT Cells in Posttransplant Alloantibody Production. American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 14(11), 2491–2499. http://doi.org/10.1111/ajt.129221600-6143https://hdl.handle.net/1805/11238We previously reported that posttransplant alloantibody production in CD8-deficient hosts is IL-4+ CD4+ T cell-dependent and IgG1 isotype-dominant. The current studies investigated the hypothesis that IL-4-producing natural killer T cells (NKT cells) contribute to maximal alloantibody production. To investigate this, alloantibody levels were examined in CD8-deficient WT, CD1d KO and Jα18 KO transplant recipients. We found that the magnitude of IgG1 alloantibody production was critically dependent on the presence of type I NKT cells, which are activated by day 1 posttransplant. Unexpectedly, type I NKT cell contribution to enhanced IgG1 alloantibody levels was interferon-γ-dependent and IL-4-independent. Cognate interactions between type I NKT and B cells alone do not stimulate alloantibody production. Instead, NKT cells appear to enhance maturation of IL-4+ CD4+ T cells. To our knowledge, this is the first report to substantiate a critical role for type I NKT cells in enhancing in vivo antibody production in response to endogenous antigenic stimuli.en-USPublisher PolicyIsoantibodiesbiosynthesisKiller Cells, NaturalimmunologyTransplantationArticle