Liu, JialingLi, YiLyu, LingnaXiao, LiangMemon, Aliza A.Yu, XinHalim, ArvinPatel, ShivaniOsman, AbdikheyreYin, WenqingJiang, JieNaini, SaidLim, KennethZhang, AifengWilliams, Jonathan D.Koester, RuthQi, Kevin Z.Fucci, Quynh-AnhDing, LaiChang, StevenPatel, AnkitMori, YutaroChaudhari, AdvikaBao, AaronLiu, JiaLu, Tzong-ShiSiedlecki, Andrew2023-11-202023-11-202022Liu J, Li Y, Lyu L, et al. Integrin α5 Is Regulated by miR-218-5p in Endothelial Progenitor Cells. J Am Soc Nephrol. 2022;33(3):565-582. doi:10.1681/ASN.2021020140https://hdl.handle.net/1805/37174Kidney endothelial cells are sensitive to hypoxic injury. This cell type expresses integrin α5 (ITGA5), which is essential to the Tie2 signaling cascade. The microRNA miR-218 is known to increase after hypoxia, but the microRNA’s role in regulating ITGA5 protein synthesis is unclear. In this study, the authors found that miR-218-5p specifically binds to ITGA5 mRNA in human kidney-derived endothelial progenitor cells (EPCs). In an animal model of ischemia/reperfusion injury, cells pretreated with an miR-218-5p mimic were delivered efficiently, whereas an animal model containing an miR-218-2 deletion specific to angioblasts resulted in kidney dysgenesis and impaired migration of mouse kidney-derived EPCs. Understanding the regulation of prominent signaling pathways in EPCs may inform optimization of therapeutic techniques for addressing kidney endothelial cell injury.en-USPublisher PolicyEndothelial cellsRenal ischemiaAcute kidney injuryArticle