Bindels, Rend J.M.Engbersen, A.M.T.Hartog, A.Blazer-Yost, Bonnie2018-08-072018-08-071996-10Rend J.M. Bindels, A.M.T. Engbersen, A. Hartog, and Bonnie Blazer-Yost. Aldosterone-induced proteins in primary cultures of rabbit renal cortical collecting system. Biochimica et Biophysica Acta (BBA) - Biomembranes. (1996).https://hdl.handle.net/1805/17019Primary cultures of immunodissected cells from rabbit kidney connecting tubule and cortical collecting duct were used to study aldosterone's action on transcellular Na+ flux. Incubation with 10(-7) M aldosterone stimulated transcellular Na+ transport which was detected as an increase in benzamil-sensitive short-circuit current. The stimulatory response was consistently noted after 2 h of incubation and stabilized after 6 h. 2D-PAGE was used to identify proteins which were induced concurrently with the increase in transcellular Na+ flux after an aldosterone incubation of 15 h. Three aldosterone-induced proteins (AIPs; M(r) = 100, 70-77 and 46-50 kDa) were found in the membrane and microsomal fractions. Two of these appeared to have more than one isoform. A single heterogeneous AIP (M(r) = 77 kDa) was detected in the soluble fraction.AldosteroneKidney CortexKidney TubulesProteinsSodiumArticle