Kim, Jun PyoCho, MinyoungKim, ChanheeLee, HyunwooJang, BeomjinJung, Sang-HyukKim, YujinKoh, In GyeongKim, SeoyeonShin, DaeunLee, Eun HyeLee, Jong-YoungPark, YoungChanJang, HyeminKim, Bo-HyunHam, HongkiKim, BeomsuKim, YujinCho, A-HyunRaj, TowfiqueKim, Hee JinNa, Duk L.Seo, Sang WonAn, Joon-YongWon, Hong-Hee2025-06-172025-06-172025-05-26Kim JP, Cho M, Kim C, et al. Whole-genome sequencing analyses suggest novel genetic factors associated with Alzheimer's disease and a cumulative effects model for risk liability. Nat Commun. 2025;16(1):4870. Published 2025 May 26. doi:10.1038/s41467-025-59949-yhttps://hdl.handle.net/1805/48777Genome-wide association studies (GWAS) on Alzheimer's disease (AD) have predominantly focused on identifying common variants in Europeans. Here, we performed whole-genome sequencing (WGS) of 1,559 individuals from a Korean AD cohort to identify various genetic variants and biomarkers associated with AD. Our GWAS analysis identified a previously unreported locus for common variants (APCDD1) associated with AD. Our WGS analysis was extended to explore the less-characterized genetic factors contributing to AD risk. We identified rare noncoding variants located in cis-regulatory elements specific to excitatory neurons associated with cognitive impairment. Moreover, structural variation analysis showed that short tandem repeat expansion was associated with an increased risk of AD, and copy number variant at the HPSE2 locus showed borderline statistical significance. APOE ε4 carriers with high polygenic burden or structural variants exhibited severe cognitive impairment and increased amyloid beta levels, suggesting a cumulative effects model of AD risk.en-USAttribution-NonCommercial-NoDerivatives 4.0 InternationalAlzheimer diseaseGenetic predisposition to diseaseWhole genome sequencingApolipoprotein E4Risk factorsArticle