Ruíz Moleón, VeraFotouhi, MaryamAlende, CharlesAyoubi, RihamBedford, Logan M.Southern, KathleenRichardson, Timothy I.Laflamme, CarlNeuroSGC/YCharOS/EDDU collaborative groupABIF consortium2024-11-122024-11-122024-01-18Ruíz Moleón V, Fotouhi M, Alende C, et al. A guide to selecting high-performing antibodies for PLC-gamma-2 for use in Western Blot, immunoprecipitation and immunofluorescence. F1000Res. 2024;13:77. Published 2024 Jan 18. doi:10.12688/f1000research.146156.1https://hdl.handle.net/1805/44487Phosphatidylinositol-specific phospholipase C gamma 2 (PLC-gamma-2) is an enzyme that regulates the function of immune cells. PLC-gamma-2 has been implicated in neurodegenerative and autoimmune disorders, yet investigation of this protein has been limited by a lack of independently characterized antibodies. Here we have characterized eleven PLC-gamma-2 commercial antibodies for use in Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.en-USAttribution 4.0 InternationalPhosphatidylinositol-specific phospholipase C gamma 2Antibody characterizationAntibody validationWestern BlotImmunoprecipitationImmunofluorescenceArticle