Teague, Walter E., Jr.Soubias, OlivierPetrache, HoriaFuller, NolaHines, Kirk G.Rand, R. PeterGawrisch, Klaus2025-05-062025-05-062013Teague WE Jr, Soubias O, Petrache H, et al. Elastic properties of polyunsaturated phosphatidylethanolamines influence rhodopsin function. Faraday Discuss. 2013;161:383-459. doi:10.1039/c2fd20095chttps://hdl.handle.net/1805/47788Membranes with a high content of polyunsaturated phosphatidylethanolamines (PE) facilitate formation of metarhodopsin-II (M(II)), the photointermediate of bovine rhodopsin that activates the G protein transducin. We determined whether M(II)-formation is quantitatively linked to the elastic properties of PEs. Curvature elasticity of monolayers of the polyunsaturated lipids 18 : 0-22 : 6(n - 3)PE, 18 : 0-22 : 5(n)- 6PE and the model lipid 18 : 1(n - 9)-18 : 1,(n- 9)PE were investigated in the inverse hexagonal phase. All three lipids form lipid monolayers with rather low spontaneous radii of curvature of 26-28 angstroms. In membranes, all three PEs generate high negative curvature elastic stress that shifts the equilibrium of MI(I)/M(II) photointermediates of rhodopsin towards M(II) formation.en-USPublisher PolicyPhosphatidylcholinesPhosphatidylethanolaminesRhodopsinCell membraneLipid bilayersArticle