Kou, QiangXun, LikunLiu, Xiaowen2018-05-092018-05-092016-11-15Kou, Q., Xun, L., & Liu, X. (2016). TopPIC: a software tool for top-down mass spectrometry-based proteoform identification and characterization. Bioinformatics, 32(22), 3495–3497. https://doi.org/10.1093/bioinformatics/btw3981367-4803https://hdl.handle.net/1805/16107Summary: Top-down mass spectrometry enables the observation of whole complex proteoforms in biological samples and provides crucial information complementary to bottom-up mass spectrometry. Because of the complexity of top-down mass spectra and proteoforms, it is a challenging problem to efficiently interpret top-down tandem mass spectra in high-throughput proteome-level proteomics studies. We present TopPIC, a tool that efficiently identifies and characterizes complex proteoforms with unknown primary structure alterations, such as amino acid mutations and post-translational modifications, by searching top-down tandem mass spectra against a protein database., Availability and Implementation: http://proteomics.informatics.iupui.edu/software/toppic/, Contact: xwliu@iupui.edu, Supplementary information: Supplementary data are available at Bioinformatics online.en-USPublisher Policymass spectrometryproteoformsamino acid mutationsProteoform Identification and CharacterizationArticle