Clendenon, Sherry G.Young, Pamela A.Ferkowicz, MichaelPhillips, CarrieDunn, Kenneth W.2016-02-222016-02-222011-08Clendenon, S. G., Young, P. A., Ferkowicz, M., Phillips, C., & Dunn, K. W. (2011). Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy. Microscopy and Microanalysis : The Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 17(4), 614–617. http://doi.org/10.1017/S14319276110005351431-9276https://hdl.handle.net/1805/8413In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.en-USPublisher PolicyFluorescenceImage Processing, Computer-AssistedmethodsKidneychemistrycytologyMicroscopy, ConfocalArticle