Srinivasan, AshokKleiman, Martin B.Debelenko, LarisaStokes, Dennis C.De Vincenzo, JohnWheat, Joseph L.2018-05-092018-05-092009-05Srinivasan, A., Kleiman, M. B., Debelenko, L., Stokes, D. C., De Vincenzo, J., & Wheat, J. L. (2009). False-negative Histoplasma antigen in acute pulmonary histoplasmosis: the value of urinary concentration by ultrafiltration and heat denaturation of serum proteins in detection of Histoplasma antigen. The Pediatric Infectious Disease Journal, 28(5), 447–449. https://doi.org/10.1097/INF.0b013e318193ecc10891-3668https://hdl.handle.net/1805/16112We report an infant with localized pulmonary histoplasmosis in whom Histoplasma antibody assays, quantitative Histoplasma urine and serum antigen concentrations, and histopathologic findings of a mediastinal mass were nondiagnostic. A provisional diagnosis of histoplasmosis was established by using laboratory methods that increase the sensitivity of the antigen assay using ultrafiltration of urine and ethylenediaminetetraacetic acid/heat denaturation of serum proteins.en-USPublisher PolicyhistoplasmosisurinaryserumantigenArticle