Damayanti, Nur P.Buno, KevinNarayanan, NagarajanHarbin, Sherry L VoytikDeng, MengIrudayaraj, Joseph M.K.2019-05-092019-05-092017-07-24Damayanti, N. P., Buno, K., Narayanan, N., Voytik Harbin, S. L., Deng, M., & Irudayaraj, J. (2017). Monitoring focal adhesion kinase phosphorylation dynamics in live cells. The Analyst, 142(15), 2713–2716. doi:10.1039/c7an00471khttps://hdl.handle.net/1805/19203Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.en-USPublisher PolicyBiosensing TechniquesCells, CulturedFluorescenceFocal Adhesion Protein-Tyrosine KinasesMicroscopy, ConfocalPhosphorylationSingle-Cell AnalysisTyrosineArticle