Agard, AmandaElsheikh, OmarBell, DrewRelich, Ryan F.Schmitt, Bryan H.Sadowski, JoshFadel, WilliamWebb, Douglas H.Dbeibo, LanaKelley, KristenCarozza, MarielLei, Guang-ShenCalkins, PaulBeeler, Cole2023-07-172023-07-172022Agard A, Elsheikh O, Bell D, et al. Clinical comparison and agreement of PCR, antigen, and viral culture for the diagnosis of COVID-19: Clinical Agreement Between Diagnostics for COVID19. J Clin Virol Plus. 2022;2(3):100099. doi:10.1016/j.jcvp.2022.100099https://hdl.handle.net/1805/34419The aim of this study is to compare the COVID-19 nasopharyngeal PCR (NP PCR) to antigen, nasal PCR, and viral culture. One-hundred-and-fourteen risk-stratified patients were tested by culture, nasal PCR, NP PCR, and Ag testing. Twenty (48%) of the high risk and 23 (32%) of the low risk were NP PCR positive. Compared with NP PCR, the sensitivity of nasal PCR, Sofia Ag, BinaxNOW Ag, and culture were 44%, 31%, 37%, and 15%. In the high risk group, the sensitivity of these tests improved to 71%, 37%, 50%, and 22%. Agreement between tests was highest between nasal PCR and both antigen tests. Patients who were NP PCR positive but antigen negative were more likely to have remote prior COVID-19 infection (p<0.01). Nasal PCR and antigen positive patients were more likely to have symptoms (p = 0.01).en-USPublisher PolicyCOVID-19SARS-CoV-2Antigen testingPCR testingViral cultureDiagnosticsArticle